Primer

Part:BBa_K4181006:Design

Designed by: Junzhi Yan   Group: iGEM22_SJTU-BioX-Shanghai   (2022-09-20)


Primer for DSUP protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 65
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 65
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 65
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers should be designed to meet the design requirements of primers


Source

atggcatccacacaccaatcatccacagaaccctcttccacaggtaaatctgaggaaacgaagaaagatgcttcgcaagggagcgggcaagactccaagaacgtaaccgttaccaaaggtaccggttcctccgccacctcagctgccattgtcaagacaggaggatcccaaggcaaagattcctctactacagcgggctcttctagtactcagggacagaagttcagtactacacctaccgacccgaaaactttcagctctgaccaaaaggagaaatccaaaagcccagccaaagaagtcccgtctggtggcgatagtaagtcccaaggtgacaccaagtctcaaagcgacgccaaatcttctggacaaagtcagggccagtctaaagacagcggcaaatcatcttccgacagtagcaagagtcactctgtcatcggagctgtcaaagacgtcgttgcaggcgccaaagatgtcgcaggaaaagccgtcgaggatgctcctagcatcatgcatactgcagtcgatgctgtgaagaacgcagccacgactgtgaaggatgtggcatcgtcggctgcatcgactgtggcggagaaggtagtcgatgcttaccacagtgtggtgggagacaagacggacgacaagaaagagggcgagcacagcggcgacaagaaggacgactccaaagctggaagtggctctggacaaggtggtgacaacaagaagtctgaaggagagacttctggccaagcagaatccagctctggcaacgaaggagctgctccagccaaaggccgtggtcgtggacggcctccagcagctgctaaaggagttgctaagggtgctgcaaagggcgctgccgcctccaaaggagccaagagcggtgctgaatcctccaagggaggagaacagtcgtcaggagatatcgagatggcagatgcttcctccaagggaggctcggaccagagggattccgcggcgaccgttggcgaaggtggtgcatcaggcagtgagggtggagctaagaaaggcagagggcggggcgctggtaagaaagcggatgcgggtgatacgtccgctgagccgcctcggcggtcgtcccgcctgacgtcttcaggtacaggggcgggttccgctccagctgcagcgaaaggcggagcgaagcgtgctgcttcttcctccagtacaccttccaacgctaagaagcaagcgactggaggtgctggcaaagctgctgccaccaaagcaactgctgccaaatcggcagcctctaaagctccccagaatggcgcaggtgccaagaagaagggaggaaaggctggaggacggaagaggaagtaa

References